Alkaline Phosphatase, Tissue N
on-Specific isozyme Polyclonal Antibody PE Conjugated[C91117PE]- 询价
- Signalway Antibody
- C91117PE
- 2025年09月22日
- FC;IF
- Rabbit
- Hu Ms Rat
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Hu Ms Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
FC;IF
- 靶点：
Uniprot：P09242
- 抗体英文名：
Alkaline Phosphatase, Tissue Non-Specific isozyme Polyclonal Antibody PE Conjugated
- 规格：
50ul/100ul
计算分子量:56
配方:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
应用详情:Flow-Cyt=1:50-200 IF=1:50-200
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
Blocking of unwanted non-specific staining
that may affect your staining. Blocking of endogenous Alkaline Phosphatase is done by exploiting the differential sensitivity of calf intestinal AP, used as a reporter, and leukocyte AP. This latter is inhibited, while the former is not by Levamisole 1 mM
Western Blotting with Alkaline Phosphatase Conjugates
Western Blotting with Alkaline Phosphatase Conjugates SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered
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