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- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against synthesized peptide derived from human CK-1 gamma1/2/3 around the phosphorylation site of Tyr263. AA range:229-278
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
IHC-p,IF(paraffin section),ELISA
- 靶点:
Uniprot:Q9HCP0
- 抗体英文名:
Casein Kinase Iγ1/2/3 (phospho-Tyr263) Polyclonal Antibody
- 规格:
50ul/100ul
计算分子量:48/48/48kd
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:
Immunohistochemistry: 1/100 - 1/300.
ELISA: 1/20000. Not yet tested in other applications.
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
of interest available; 2) formation of the antibody-antigen complex by adding specific antibody; 3) separation and detection of the immune complex by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Both polyclonal and monoclonal
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