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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against synthesized peptide derived from human GRK2 around the phosphorylation site of Ser29. AA range:14-63
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB,IHC-p,IF(paraffin section),ELISA
- 靶点:
Uniprot:P25098
- 抗体英文名:
GRK 2 (phospho-Ser29) Polyclonal Antibody
- 规格:
50ul/100ul
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
ELISA: 1/10000. Not yet tested in other applications.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
), and causes activation of KOR. Agonist-activated KOR becomes a substrate for G protein receptor kinase (GRK), which phosphorylates the Ser369 residue at the C-terminal tail of the receptor in the first step in the β-Arrestin-dependent desensitization cascade
Synthesis and Probing of Membrane-bound Peptide Arrays
in the presence of probe antibody (or protein) diluted in 8-10 ml of MBS. For monoclonal antibodies, or pure proteins, use ~4-5 µg of purified antibody per milliliter of incubation volume. When using a polyclonal serum, we recommend a dilution
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