- 询价
- Signalway Antibody
- 13416
- 2026年05月21日
- WB;ICC/IF;IHC
- Recombinant Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic phospho-peptide corresponding to residues surrounding Thr401 of human B Raf.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB;ICC/IF;IHC
- 靶点：
Uniprot：P15056
- 抗体英文名：
B Raf(Phospho-T401) Rabbit mAb
- 规格：
50ul/100ul
94 kDa B raf protein antibody
B raf 1 antibody
B Raf proto oncogene serine threonine protein kinase antibody
B Raf proto oncogene, serine/threonine kinase antibody
B RAF1 antibody
B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody
BRAF 1 antibody
BRAF antibody
BRAF_HUMAN antibody
BRAF1 antibody
cRmil antibody
MGC126806 antibody
MGC138284 antibody
Murine sarcoma viral (v-raf) oncogene homolog B1 antibody
Murine sarcoma viral v raf oncogene homolog B1 antibody
NS7 antibody
Oncogen BRAF antibody
oncogene BRAF1 antibody
p94 antibody
Proto-oncogene B-Raf antibody
Proto-oncogene c-Rmil antibody
RAFB 1 antibody
RAFB1 antibody
RMIL antibody
Serine/threonine-protein kinase B-raf antibody
v raf murine sarcoma viral oncogene homolog B antibody
v raf murine sarcoma viral oncogene homolog B1 antibody
v-Raf murine sarcoma viral oncogene homolog B1 antibody
计算分子量:84 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
图片:
Western blot analysis of Phospho-B Raf(T401) on different lysates using anti-Phospho-B Raf(T401) antibody at 1/1,000 dilution. Positive control:
Lane 1: Mouse testis
Lane 2: PC-12
,
ICC staining Phospho-B Raf(T401) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining Phospho-B Raf(T401) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining Phospho-B Raf(T401) in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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文献和实验Monitoring Oncogenic B-RAF-Induced Senescence in Melanocytes
The B-RAF kinase is a downstream effector of the RAS family of proto-oncogenes and is constitutively activated in the majority of human melanomas. The common oncogenic B-RAFV600E mutant cooperates with additional genetic lesions to transform
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
Testing Rabbit Bleeds By Elisa
same amount of NaBH4 after 15''. Do a quick microfuge spin if there are too many bubbles. B. ELISA with Peptide conjugated to BSA Coat 10 ug/ml antigen (peptide conjugated to BSA) diluted in TBS (50 ul/well) ON at 4 deg C. Remove antigen
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