- 询价
- Signalway Antibody
- 49928
- 2025年09月23日
- WB,ICC,IF,IP,IHC,FC
- Recombinant Rabbit
- Human;Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein within human PIST aa 1-100.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal antibody
- 适应物种：
Human;Mouse
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB,ICC,IF,IP,IHC,FC
- 靶点：
Uniprot：Q9HD26
- 抗体英文名：
PIST Rabbit mAb
- 规格：
50ul/100ul
CFTR associated ligand antibody
CFTR-associated ligand antibody
dJ94G16.2 antibody
dJ94G16.2 PIST antibody
FIG antibody
Fused in glioblastoma antibody
Golgi associated PDZ and coiled coil motif containing antibody
Golgi associated PDZ and coiled coil motif containing protein antibody
Golgi-associated PDZ and coiled-coil motif-containing protein antibody
GOPC 1 antibody
GOPC antibody
GOPC_HUMAN antibody
GOPC1 antibody
OTTHUMP00000040403 antibody
PDZ protein interacting specifically with TC 10 antibody
PDZ protein interacting specifically with TC10 antibody
PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody
PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody
PIST antibody
Protein interacting specifically with Tc 10 antibody
Protein interacting specifically with Tc10 antibody
计算分子量:51 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:10-1:50
FC: 1:50-1:100
图片:
Western blot analysis of PIST on SiHa cell using anti-PIST antibody at 1/1,000 dilution.
,
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PIST antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PIST antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PIST antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PIST antibody. Counter stained with hematoxylin.
,
ICC staining PIST in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining PIST in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining PIST in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
Flow cytometric analysis of MCF-7 cells with PIST antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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