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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal antibody
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Recombinant Rabbit
- 应用范围:
WB,ICC,IF,IP,IHC,FC
- 靶点:
Uniprot:O43707
- 抗体英文名:
alpha Actinin 4 Rabbit mAb
- 规格:
50ul/100ul
Actinin alpha 4 antibody
actinin4 antibody
ACTN 4 antibody
ACTN4 antibody
ACTN4_HUMAN antibody
Alpha-actinin-4 antibody
DKFZp686K23158 antibody
F actin cross linking protein antibody
F-actin cross-linking protein antibody
Focal segmental glomerulosclerosis 1 antibody
FSGS 1 antibody
FSGS antibody
FSGS1 antibody
Non muscle alpha actinin 4 antibody
Non-muscle alpha-actinin 4 antibody
计算分子量:105 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500
IP: 1:10-1:50
FC: 1:50-1:100
图片:

Western blot analysis of alpha Actinin 4 on different lysates using anti-alpha Actinin 4 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: NIH-3T3
Lane 4: Rat liver tissue
Lane 5: A431
Lane 6: HepG2
,

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin.
,

ICC staining alpha Actinin 4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,

ICC staining alpha Actinin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,

ICC staining alpha Actinin 4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,

Flow cytometric analysis of A549 cells with alpha Actinin 4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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文献和实验Immunohistochemistry / Immunocytochemistry Procedures
of fixative for each antigen. However frozen sections give less morphological detail and resolution than other methods. Rabbit anti-alpha-internexin (Cat No. AB5354
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
.1 ) Phosphate‐buffered saline (PBS; Fisher Scientific) Alexa Fluor 546–conjugated goat anti—rabbit IgG (Invitrogen, cat. no. A‐11035
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