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文献和实验The Cell Environment (including types of culture medium)
free (Prod. No. W3500, water, tissue culture grade). In most cases water prepared by reverse osmosis and resin cartridge purification with a final resistance of 16-18Mx is suitable. Once prepared the media should be filter sterilized before use
Differentiate ES cells into glial cells and neurons
together by gentle handling. Then directly plate the cells 1:3 into bacterial grade Petri dishes in LIF free medium containing 1 microM all-trans retinoic acid (RA).Day 3 Collect cell aggregates and replate in tissue culture dishes (appr. 25 cell aggregates per 6 cm
The Cell Environment (including types of culture medium)
which supports high density growth Leibovitz L-15 (Prod. No. L5520 , liquid) Designed for CO2 free environments TC 100 (Prod. No. T3160 ) Grace's Insect Medium (Prod
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