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- 询价
- Signalway Antibody
- 13373
- 2026年05月21日
- WB;ICC/IF;IHC
- Recombinant Rabbit
- Human
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- 文献和实验
- 技术资料
- 免疫原：
Synthetic phospho-peptide corresponding to residues surrounding Ser218 and 222 of human MEK1.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB;ICC/IF;IHC
- 靶点：
Uniprot：Q02750
- 抗体英文名：
MEK1(Phospho-S218/S222) Rabbit mAb
- 规格：
50ul/100ul
Dual specificity mitogen-activated protein kinase kinase 1 antibody
ERK activator kinase 1 antibody
MAP kinase kinase 1 antibody
MAP kinase/Erk kinase 1 antibody
MAP2K1 antibody
MAPK/ERK kinase 1 antibody
MAPKK 1 antibody
MAPKK1 antibody
MEK 1 antibody
Mek1 antibody
MEKK1 antibody
Mitogen activated protein kinase kinase 1 antibody
MKK 1 antibody
MKK1 antibody
MP2K1_HUMAN antibody
PRKMK1 antibody
Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
Protein kinase mitogen activated, kinase 1 antibody
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计算分子量:45 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
图片:
Western blot analysis of Phospho-MEK1(S218/S222) on different lysates using anti-Phospho-MEK1(S218/S222) antibody at 1/1,000 dilution. Positive control:
Lane 1: A431
Lane 2: Hela
Lane 3: 293T
,
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-MEK1(S218/S222) antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Phospho-MEK1(S218/S222) antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-MEK1(S218/S222) antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Phospho-MEK1(S218/S222) antibody. Counter stained with hematoxylin.
,
ICC staining Phospho-MEK1(S218/S222) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining Phospho-MEK1(S218/S222) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining Phospho-MEK1(S218/S222) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
CST 抗体 9 月特惠｜购买任意 CST 抗体，+168 元即可入手 25μl 标签 / 内参抗体
HRP、Alexa Fluor® 488、Alexa Fluor® 594 等多种标记一抗可选。 热门可选产品参考 货号 产品名称 类型 加价购价格 S0B0005-25u1 B-actin Rabbit mAb (SDT-RO15) 裸抗 168 元 S0B0004-25u1 B-tubulin Recombinant Rabbit mAb (SDT-RO14) 裸抗 168 元 S0B0383-25u1 S-RMab® Myc tag Recombinant Rabbit
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
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