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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic phospho-peptide corresponding to residues surrounding Ser209 of human eIF4E.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Recombinant Rabbit
- 应用范围:
WB;ICC/IF;IHC
- 靶点:
Uniprot:P06730
- 抗体英文名:
eIF4E (Phospho-S209) Rabbit mAb
- 规格:
50ul/100ul
CBP antibody
eIF 4E antibody
eIF 4F 25 kDa subunit antibody
EIF 4F antibody
eIF-4E antibody
eIF-4F 25 kDa subunit antibody
eIF4E antibody
EIF4E1 antibody
EIF4EL1 antibody
EIF4F antibody
Eukaryotic translation initiation factor 4 E antibody
Eukaryotic translation initiation factor 4E antibody
Eukaryotic translation initiation factor 4E like 1 antibody
IF4E_HUMAN antibody
Messanger RNA Cap Binding Protein eIF 4E antibody
MGC111573 antibody
mRNA cap binding protein antibody
mRNA cap-binding protein antibody
计算分子量:25 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
图片:

Western blot analysis of Phospho-eIF4E(S209) on different lysates using anti-Phospho-eIF4E(S209) antibody at 1/1,000 dilution. Positive control:
Lane 1: Mouse spleen
Lane 2: Rat spleen
,

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-eIF4E(S209) antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Phospho-eIF4E(S209) antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Phospho-eIF4E(S209) antibody. Counter stained with hematoxylin.
,

ICC staining Phospho-eIF4E(S209) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,

ICC staining Phospho-eIF4E(S209) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,

ICC staining Phospho-eIF4E(S209) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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文献和实验:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
david9931 请问各位大侠,有没有订做过磷酸化的丝氨酸或磷酸化的苏氨酸抗体,哪家公司做的比较好,小弟想订做目的蛋白特异位点的丝氨酸、苏氨酸的磷酸化的抗体,帮忙给指点下,多谢啦 euthor_hsu 这种抗体定做很难。毕竟要的是抗一个特异的氨基酸的磷酸化位点。 就我所知cisbio有anti-phosphothronine 和anti-phosphotyrosine的抗体,PAb和MAb都有,但是没有anti
。所以,我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体:Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本:石蜡包埋人类肺部肿瘤样本煮沸设备:浴锅 (左),微波炉 (中),高压锅 (右)免疫染色—封闭在 IHC–P 中,我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品,时长 1 小时,以防止非特异性背景染色。在进行 IHC–F 时,在含有 0.3% Triton. X–100 的 1X
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