- 询价
- Signalway Antibody
- 13362
- 2025年09月23日
- WB;ICC/IF;IHC
- Recombinant Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic phospho-peptide corresponding to residues surrounding Thr359 and Ser363 of human RSK1 p90
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB;ICC/IF;IHC
- 靶点：
Uniprot：Q15418
- 抗体英文名：
RSK1 p90(Phospho-T359/S363) Rabbit mAb
- 规格：
50ul/100ul
dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
dJ590P13.1 antibody
EC 2.7.11.1 antibody
HU 1 antibody
HU1 antibody
KS6A1_HUMAN antibody
MAP kinase activated protein kinase 1a antibody
MAP kinase-activated protein kinase 1a antibody
MAPK-activated protein kinase 1a antibody
MAPKAP kinase 1a antibody
MAPKAPK-1a antibody
MAPKAPK1A antibody
MGC79981 antibody
Mitogen-activated protein kinase-activated protein kinase 1A antibody
OTTHUMP00000004113 antibody
p90 RSK1 antibody
p90-RSK 1 antibody
p90rsk antibody
p90RSK1 antibody
p90S6K antibody
pp90RSK1 antibody
Ribosomal protein S6 kinase 90kD 1 antibody
Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
Ribosomal protein S6 kinase alpha 1 antibody
Ribosomal protein S6 kinase alpha-1 antibody
Ribosomal protein S6 kinase polypeptide 1 antibody
Ribosomal S6 kinase 1 antibody
RPS6K1 alpha antibody
rps6ka antibody
Rps6ka1 antibody
RSK 1 antibody
RSK 1 p90 antibody
RSK antibody
RSK-1 antibody
RSK1 antibody
S6K alpha 1 antibody
S6K-alpha-1 antibody
计算分子量:83 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
图片:
Western blot analysis of Phospho-RSK1 p90(T359+S363) on different lysates using anti-Phospho-RSK1 p90(T359+S363) antibody at 1/1,000 dilution. Positive control:
Lane 1: A431
Lane 2: 293
Lane 3: MCF-7
,
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-RSK1 p90(T359+S363) antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-RSK1 p90(T359+S363) antibody. Counter stained with hematoxylin.
,
ICC staining Phospho-RSK1 p90(T359+S363) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining Phospho-RSK1 p90(T359+S363) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining Phospho-RSK1 p90(T359+S363) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
david9931 请问各位大侠，有没有订做过磷酸化的丝氨酸或磷酸化的苏氨酸抗体，哪家公司做的比较好，小弟想订做目的蛋白特异位点的丝氨酸、苏氨酸的磷酸化的抗体，帮忙给指点下，多谢啦 euthor_hsu 这种抗体定做很难。毕竟要的是抗一个特异的氨基酸的磷酸化位点。 就我所知cisbio有anti-phosphothronine 和anti-phosphotyrosine的抗体，PAb和MAb都有，但是没有anti
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
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