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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Cytokeratin 20 Rabbit mAb
- 抗体英文名:
Cytokeratin 20 Rabbit mAb
- 应用范围:
WB, ICC/IF, IHC, IP, FC
- 宿主:
Recombinant Rabbit
- 适应物种:
Human;Rat
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 保存条件:
Store at -20°C
- 免疫原:
recombinant protein
purification
ProA affinity purified
applications
WB, ICC/IF, IHC, IP, FC
appl_detail
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:10-1:100
other_names
CD20 antibody
CK 20 antibody
CK-20 antibody
CK20 antibody
Cytokeratin-20 antibody
Cytokeratin20 antibody
K1C20_HUMAN antibody
K20 antibody
KA20 antibody
Keratin 20 antibody
keratin 20, type I antibody
keratin 21, rat, homolog of antibody
Keratin antibody
Keratin type I cytoskeletal 20 antibody
Keratin-20 antibody
Keratin20 antibody
KRT 20 antibody
KRT 21 antibody
KRT20 antibody
KRT21 antibody
MGC35423 antibody
OTTHUMP00000164518 antibody
Protein IT antibody
type I cytoskeletal 20 antibody
calculated_mw
44 kDa
formulation
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
uniprot
P35900
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文献和实验mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
2 hours to coat antibody to flask or plate 20 minutes preparation of spleen single cell suspension 20 minutes to set up the assay 2-4 days incubation Method Antibody Coating of the Assay Plate Microwells: Prepare a 5-10
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