- 询价
- Signalway Antibody
- 48619
- 2025年09月23日
- WB, ICC/IF, IHC, IP
- Recombinant Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
recombinant protein
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal antibody
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB, ICC/IF, IHC, IP
- 靶点：
Uniprot：P24385
- 抗体英文名：
Cyclin D1 Rabbit mAb
- 规格：
50ul/100ul
B cell CLL/lymphoma 1 antibody
B cell leukemia 1 antibody
B cell lymphoma 1 protein antibody
B-cell lymphoma 1 protein antibody
BCL 1 antibody
BCL-1 antibody
BCL-1 oncogene antibody
BCL1 antibody
BCL1 oncogene antibody
ccnd1 antibody
CCND1/FSTL3 fusion gene, included antibody
CCND1/IGHG1 fusion gene, included antibody
CCND1/IGLC1 fusion gene, included antibody
CCND1/PTH fusion gene, included antibody
CCND1_HUMAN antibody
cD1 antibody
Cyl 1 antibody
D11S287E antibody
G1/S specific cyclin D1 antibody
G1/S-specific cyclin-D1 antibody
Parathyroid adenomatosis 1 antibody
PRAD1 antibody
PRAD1 oncogene antibody
U21B31 antibody
计算分子量:34 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
图片:
Western blot analysis of Cyclin D1 on different lysates using anti-Cyclin D1 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: SH-SY-5Y
,
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
,
ICC staining Cyclin D1 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining Cyclin D1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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文献和实验Immunodetection of cyclin D1 and D2/D3 using flow cytometry
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E Procedure Prepare the cell type of interest as a single cell suspension and wash once with cold PBS
Disorders of the cell cycle regulatory machinery play a key role in the pathogenesis of cancer. The gene encoding the cyclin D1 protein, a regulator of the progression from the G1- to S-phase, is often found disrupted in the cancer cell
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E, and is based on protocols originally designed by Z. Darzynkiewicz and colleagues. Although FITC-conjugated
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