- 询价
- Signalway Antibody
- 13329
- 2025年09月23日
- WB, IP, IF, IHC(P)
- Mouse
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A sequence containing Ser 411 phosphorylated p70 S6 kinase α isoform 2 of human origin.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB, IP, IF, IHC(P)
- 靶点：
Uniprot：Q9UBS0
- 抗体英文名：
p70 S6 Kinase (Phospho-Ser 411) Antibody
- 规格：
50ul/100ul
EC 2.7.11.1 antibody
KS6B2_HUMAN antibody
p70 beta antibody
p70 ribosomal S6 kinase beta antibody
p70 S6 kinase beta antibody
p70 S6K-beta antibody
p70 S6KB antibody
p70 S6Kbeta antibody
p70(S6K) beta antibody
p70-beta antibody
p70-S6K 2 antibody
P70S6K2 antibody
p70S6Kb antibody
Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
Ribosomal protein S6 kinase beta 2 antibody
Ribosomal protein S6 kinase beta-2 antibody
Rps6kb2 antibody
S6 kinase related kinase antibody
S6 kinase-related kinase antibody
S6K beta 2 antibody
S6K beta antibody
S6K-beta antibody
S6K-beta-2 antibody
S6K2 antibody
Serine/threonine protein kinase 14 beta antibody
Serine/threonine-protein kinase 14B antibody
SRK antibody
STK14B antibody
计算分子量:70kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:
WB: 1:100-1:1,000
IHC: 1:50-1:500
IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
图片:
Western blot analysis of p70 S6 kinase α phosphorylation in untreated (A,D), insulin treated (B,E) and insulin treated and lambda protein phosphatase treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-p70 S6 kinase α (A-6) (A,B,C) and p70 S6 kinase α (C-18) (D,E,F).
,
Immunofluorescence staining of anisomycin-treated, methanol-fixed NIH/3T3 cells, showing cytoplasmic and nuclear localization of activated p70 S6 kinase α (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of squamous epithelial cells (B).
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
ERK-MAP Kinase Signaling in the Cytoplasm
. We describe two complementary methods for quantitatively measuring ERK activity toward the cytoplasmic p90 ribosomal S6 kinase (RSK). The first method is a straightforward immunoblot of endogenous ERK and RSK phosphoepitopes using phospho-specific antibodies. Infrared
Measurement of Phosphoinositide 3-Kinase Activity
only PtdIns as a substrate (e.g., mammalian PtdIns 3-kinase and yeast Vps34p) ( 1 ). Generally, PI 3 kinases are now regarded as an important intracellular signal upstream of a variety of biochemical (e.g., activation of Akt/protein kinase B and/or p70
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