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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
peptide
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Mouse
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Mouse
- 应用范围:
WB, IP, IF, IHC(P)
- 靶点:
Uniprot:Q05397
- 抗体英文名:
FAK (Phospho-Ser 722) Antibody
- 规格:
50ul/100ul
FADK antibody
FAK related non kinase polypeptide antibody
FAK1 antibody
FAK1_HUMAN antibody
Focal adhesion kinase 1 antibody
Focal adhesion Kinase antibody
Focal adhesion kinase isoform FAK Del33 antibody
Focal adhesion kinase related nonkinase antibody
FRNK antibody
p125FAK antibody
pp125FAK antibody
PPP1R71 antibody
Protein phosphatase 1 regulatory subunit 71 antibody
Protein tyrosine kinase 2 antibody
Protein-tyrosine kinase 2 antibody
Ptk2 antibody
PTK2 protein tyrosine kinase 2 antibody
计算分子量:125 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:
WB: 1:100-1:1,000
IHC: 1:50-1:500
IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
图片:

A.Western blot analysis of FAK phosphorylation in non-transfected:(A,D), untreated human FAK transfected:(B,E) and lambda protein phosphatase treated human FAK transfected:(C,F) 293T whole cell lysates. Antibodies tested include p-FAK (A-12):(A,B,C) and FAK (C-903):(D,E,F).
,

B.Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.
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文献和实验Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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