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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
peptide
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Mouse
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Mouse
- 应用范围:
WB, IP, IF
- 靶点:
Uniprot:Q13541
- 抗体英文名:
4E-BP1/2/3 (Phospho-Thr 45) Antibody
- 规格:
50ul/100ul
4EBP1 antibody
4EBP1_HUMAN antibody
BP 1 antibody
eIF4E binding protein 1 antibody
eIF4E-binding protein 1 antibody
Eif4ebp1 antibody
Eukaryotic translation initiation factor 4E-binding protein 1 antibody
PHAS-I antibody
PHASI antibody
Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
计算分子量:21 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
图片:

Westernblot analysis of 4E-BP1/2/3 phosphorylation inuntreated (A), calyculinA treated (B) and calyculin A and lambda protein phosphatase treated (C) Jurkat whole cell lysates.
,

Western blot analysis of 4E-BP1 phosphorylation in nontransfected (A,D), untreated human 4E-BP1 transfected (B,E) and lambda protein phosphatase treated human 4E-BP1 transfected (C,F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A,B,C) and 4E-BP1 (D,E,F).
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文献和实验CD45/CD45RA/CD45RB/CD45RC/CD45RO
CD45/CD45RA/CD45RB/CD45RC/CD45RO CD45 常用单克隆抗体或代号: T29/33,BMAC1;(T200,B220) 主要表达细胞: Leu [NL] 分子质量(kDa)和结构: gp180- 240,白细胞共同抗原(LCA) 功 能: PTP酶,调节信号传导 CD45RA 常用单克隆抗体或代号: G1- 15,F8- 11- 13,Leu18,2H4;(限制性LCA) 主要表达细胞: Tsub,B,G,M
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
slurry (Fisher Scientific 45‐000‐116) Immunoprecipitation (IP) wash buffer (see recipe ) Phospho‐motif antibody
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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