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- 详细信息
- 文献和实验
- 技术资料
- 服务名称:
Recombinant Hal l 1 protein
- 提供商:
普健生物(武汉)科技有限公司
Uniprot号:Q90XF2
基因名:Hal l 1
蛋白名:Protein kinase C iota type
蛋白别名:
hal
服务内容:
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示:

Hal l 1相关研究文献:
1. Real-World Effectiveness of IL-5/5Ra Targeted Biologics in Severe Eosinophilic Asthma With Comorbid Bronchiectasis. [37295671]
2. The perceived waning of biologics in severe asthma. [37757988]
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文献和实验Purification of recombinant sBRF M166L
culture with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml) and grow at 30 deg. for four hours. Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g
Purification of recombinant sBRF M166L
with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml)and grow at 30 deg.for four hours.Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g).Resuspend the cell
Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography
(five or six His residues) placed at either the C- or N-terminus of a recombinant protein can form a stable chelate with immobilized transition metals. This allows fractionation of the target protein to 90–95% purity levels in a single chromatographic step (2 –4 ). Metals
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