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- 详细信息
- 文献和实验
- 技术资料
- 提供商:
吉满/Genomeditech
- 服务名称:
重组DT3C 蛋白 (Cat.GM-046001RP)
- 规格:
询价
产品规格
GM-046001RP-1:100 μg
GM-046001RP-2:1 mg
产品简介
吉满生物重组DT3C 蛋白 (Cat.GM-046001RP) 从大肠杆菌中(E.coli)表达,包含33-417aa (P00588) & 291-497aa (P19909),8´His标签序列置于DT3C编码序列的N端,纯度≥90% (SDS-PAGE),分子量69.4 kDa,溶解在pH7.4的PBS中,经过 0.2 μm 过滤膜除菌,内毒素< 1EU/μg。
吉满生物重组DT3C蛋白使用非常简便,将DT3C蛋白和目的抗体孵育30 min即可形成mAb-DT3C共轭物。该共轭物可直接加到完全培养基的细胞中进行孵育。其在体外的功能类似于抗体药物共轭物(ADC),将白喉毒素的细胞毒性效应通过内化作用有针对性地传递到靶细胞中,评估细胞对mAb内化效率。
数据验证


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文献和实验Protein C (PC) is a 62-kDa vitamin K-dependent plasma zymogen which, after activation to serine protease, plays an important role in the physiologic regulation of blood coagulation. Given that PC is one of the major naturally
Expression of Recombinant Cytochromes c in E. coli
for proper folding and stability. However, significant advances in expression of recombinant cytochromes c have been made during the last decade. It has been shown that a single gene cluster, ccmA–H , is responsible for cytochrome c maturation in Escherichia
. This purification process resulted in greater than 95% pure rBoNTC(Hc )-C based on SDS-PAGE, and yielded up to 0.2 g of rBoNTC(Hc )-C/kg cells. The final protein yield is a function of protein expression level during fermentation and the purification methods
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