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- 湖北省武汉市
- Recombinant Asc l 13 protein
- 2025年09月10日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
Recombinant Asc l 13 protein
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：Q9ULZ3
基因名：Asc l 13
蛋白名：Apoptosis-associated speck-like protein containing a CARD
蛋白别名：
TMS1, ASC, CARD5
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
Asc l 13相关研究文献：
1. Oral Nirmatrelvir for High-Risk, Nonhospitalized Adults with Covid-19. [35172054]
2. Cryo-EM structures of the active NLRP3 inflammasome disc. [36442502]
3. Second-line FOLFOX chemotherapy versus active symptom control for advanced biliary tract cancer (ABC-06): a phase 3, open-label, randomised, controlled trial. [33798493]
4. Parasite allergens. [29588070]
5. MRE11 liberates cGAS from nucleosome sequestration during tumorigenesis. [38200309]
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文献和实验Isolation of Recombinant MVA Using F13L Selection
. This chapter describes the use of an efficient and easy-to-use selection system adapted for MVA. The system is based on the requirement of the viral gene F13L for efficient virus spread in cell culture, which results in a severe block in virus transmission
Purification of recombinant sBRF M166L
culture with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml) and grow at 30 deg. for four hours. Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g
Purification of recombinant sBRF M166L
with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml)and grow at 30 deg.for four hours.Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g).Resuspend the cell
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