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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized peptide derived from human Beclin-1 around the phosphorylation site of Ser93/96.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 供应商:
南京赛戈巍生物科技有限公司
- 应用范围:
WB
- 靶点:
Uniprot:Q14457
- 抗体英文名:
Beclin-1 (Phospho-Ser93/96) Antibody
- 规格:
50ul/100ul
计算分子量:60KD
配方:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
Western blotting: 1:1000-3000
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号;因此,我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂,我们则建议使用与二抗来源相同的血清进行封闭。抗体:Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本:石蜡
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