- 询价
- Signalway Antibody
- 12113
- 2025年09月22日
- WB
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of serine 250 (D-E-S(p)-D-D) derived from Human Proteasome α3.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
Uniprot：P25788
- 抗体英文名：
Proteasome α3 (Phospho-Ser250) Antibody
- 规格：
50ul/100ul
配方:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Western blotting: 1:500~1:3000
图片:
Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 30mins), using Proteasome α3 (Phospho-Ser250) antibody #12113. The lane on the right is treated with the synthesized peptide.
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文献和实验Studies of the Ubiquitin Proteasome System
(see recipe ) 40 mM sodium 2‐oxoglutarate (α‐ketoglutarate, sodium salt; Sigma) 40 mM ascorbic acid (Sigma) Anti‐MBP antibody (Santa Cruz Biotechnology) Boiling water bath
, members of a new family of threonine (Thr)-proteases. The outer rings, made of α-subunits, bind the regulators that control the substrate specificity of the proteasome. The binding of a 19S regulator to each end of the 20S core creates the 26S proteasome
each of seven different α and β subunits arranged into four stacked rings (α7 β7 β7 α7 ). Separation by two-dimensional (2D) gel electrophoresis of the human erythrocytes 20S proteasome subunits and mass spectrometry (MS) identification of all the observed spots reveal
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