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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of serine 662 (A-E-S(p)-V-A) derived from Human Period Circadian Protein 2.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB IHC
- 靶点:
Uniprot:O15055
- 抗体英文名:
Period Circadian Protein 2 (Phospho-Ser662) Antibody
- 规格:
50ul/100ul
配方:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
图片:

Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using Period Circadian Protein 2 (Phospho-Ser662) antibody #12112. The lane on the right is treated with the synthesized peptide.
,

Western blot analysis of extracts from 3T3£?HeLa and K562 cells, using Period Circadian Protein 2 (Phospho-Ser662) Antibody #12112. The lane on the left is treated with synthesized peptide.
,

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Period Circadian Protein 2 (Phospho-Ser662) antibody #12112. The picture on the right is treated with the synthesized peptide.
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文献和实验Manipulation of Mammalian Cell Lines for Circadian Studies
In mammals, the central circadian pacemaker resides in the hypothalamic suprachiasmatic nucleus (SCN), but circadian oscillators also exist in peripheral tissues. We have used wild-type and cryptochrome (m Cry)-deficient mouse embryonic
Probing Circadian Rhythms in Chlamydomonas rheinhardtii by Functional Proteomics
) before the cells are released into constant conditions of dim light and temperature. Under these free-running conditions circadian rhythms will continue with a period of about 24 h and cells can be harvested during specific time-points of subjective day and night
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
INTRODUCTION The formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae
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