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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic acetylated peptide corresponding to residues surrounding Lys27 of human histone H3.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat;Monkey;Arabidopsis thaliana
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB;IHC;IF;IP;ELISA
- 靶点:
Uniprot:P68431
- 抗体英文名:
Histone H3(Acetyl-Lys27) Rabbit Polyclonal Antibody
- 规格:
50ul/100ul
配方:PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
应用详情:WB 1:500-1:2000;
IHC 1:100-1:300;
IF 1:200-1:1000;
IP 1:200;
ELISA 1:5000
图片:

Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using #HW077 diluted at 1:1,000.
,

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 (Acetyl-Lys27) Antibody. The picture on the right is blocked with the synthesized peptide.
,

Immunofluorescence analysis of Hela cell. Histone H3 (Acetyl Lys27) Polyclonal Antibody(red) was diluted at 1:200(4° overnight).
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文献和实验Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
should also be included. In Ringrose et al ., (2004) I used histone H3 peptides spanning residues 1-13 (for lysine 4 and lysine 9 modifications) or 21-33 (for lysine 27 modifications). Stock solutions of 0.5mM peptide in PBS can be prepared and kept at -20°C
IHC IC2575Acetyl-Histone H2B (Lys12) AntibodyRabbitW IHC IC2571Acetyl-Histone H2B (Lys20) AntibodyRabbitW IP IHC IC2574Acetyl-Histone H2B (Lys5) AntibodyRabbitW IP IHC IC9675Acetyl-Histone H3 (Lys18) AntibodyRabbitW IHC IC9674Acetyl-Histone H3 (Lys23
," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
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