- 询价
- Signalway Antibody
- 35512
- 2025年09月22日
- WB
- Rabbit
- Human;Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 72 and 377 of alpha Actin.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
Uniprot：P68032
- 抗体英文名：
alpha Actin (skeletal muscle) Antibody
- 规格：
50ul/100ul
配方:Rabbit IgG in 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
应用详情:Western blotting: 1:500-1:3000
图片:
Sample (30 ug of whole cell lysate)
A: A549
B: HeLa
10% SDS PAGE
#35512 diluted at 1:1000
,
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
#35512 diluted at 1:1000
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文献和实验Preparation of Conventional Actin from Skeletal Muscle
Modified from Spudich & Watt, 1971, JBC 246:4866. 1. Mix 20 ml buffer G with each gm of muscle acetone powder. Extract with stirring on ice for 30 min. Use a big stir bar or overhead stirrer. Buffer G: 0.2 mM ATP, 0.5 mM DTT, 0.2 mM
by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
Protein purification: skeletal muscle myosin
Overview SKELETAL MUSCLE MYOSIN A two-headed hexomeric actin-activated ATPase consisting of two heavy chains of ~200 kDa and four light chains of ~20kDa. The tail portion
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