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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S(p)-L-C) derived from Human IKK-alpha/beta.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB,IHC,IF,ELISA
- 靶点:
Uniprot:O15111/O14920
- 抗体英文名:
IKK- alpha/ beta (Phospho-Ser176/177) Antibody
- 规格:
50ul/100ul
配方:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
Western blotting: 1:500~1:1000
图片:

All Lanes:IKK- alpha/ beta (Phospho-Ser176/177) Antibody at 1/ 500 dilution.Lane1:Jurkat treated with 20ng/ml TNF-α for 30min Cell lysateLane2: Huvec treated with 100ng/ml EGF for 30min Cell lysateLane3: Hela treated with 100ng/ml EGF for 30min Cell lysateLane4: HepG2 treated with 100ng/ml EGF for 30min Cell lysateLane5: Mouse Brain Tissue lysateLane6:Mouse lung Tissue lysateLane7:Rat Liver Tissue lysateLysates/proteins at 40 μg per lane. Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 85kDaObserved band size:?80kDa
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文献和实验下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
or HP1s associated with 5' biotin labeled siRNA EF52. Schematic methodology is shown for detection from cell lysates of biotin labeled siRNAs in complex with flag-tagged DNMTs or HP1s (alpha, beta, or gamma). B) Triple immunoprecipitation assay
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