- 询价
- Signalway Antibody
- 11582
- 2025年09月04日
- WB IHC IF
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around Tri-Methylation site of lysine 27(A-R-K(tri-methyl)-S-A) derived from Human Histone H3.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB IHC IF
- 靶点：
Uniprot：P68431
- 抗体英文名：
Histone H3 (Tri-Methyl-Lys27) Antibody
- 规格：
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
Predicted MW: 17kd
Western Blot: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
图片:
Western blot analysis of extracts from HT29 and C6 cells using Histone H3 (Tri-Methyl-Lys27) Antibody #11582.
,
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3 (Tri-Methyl-Lys27) Antibody#11582.
,
Immunofluorescence staining of methanol-fixed MEF cells using Histone H3 (Tri-Methyl-Lys27) Antibody #11582.
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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
Genome-Wide Measurement of Histone H3 Replacement Dynamics in Yeast
chromatin. Understanding the dynamic behavior of chromatin is of great interest for fields ranging from transcriptional regulation through meiosis and gametogenesis. Here, we describe a protocol for measuring histone replacement rates genome wide
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