- 询价
- Signalway Antibody
- 11247
- 2025年09月04日
- WB IHC IF
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of serine 78 (A-L-S(p)-R-Q) derived from Human HSP27.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB IHC IF
- 靶点：
Uniprot：P04792
- 抗体英文名：
HSP27(Phospho-Ser78) Antibody
- 规格：
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
图片:
Western blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27(Phospho-Ser78) Antibody #11247.
,
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Phospho-Ser78) Antibody #11247(left) or the same antibody preincubated with blocking peptide(right).
,
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27(Phospho-Ser78) Antibody #11247.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人热休克蛋白27 ( HSP27 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 HSP27 单抗包被于酶标板上，标准品和样品中的 HSP27与单抗结合，加入生物素化的抗人 HSP27 ，形成免疫复合物连接在板上，辣根
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
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