p44/42 MAP Kinase(Phospho-Tyr2

别名:ERK1, ERT2, ERK-1, PRKM3, P44ERK1
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:

Predicted MW: 42 44 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

图片:

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody #11246 (left) or the same antibody preincubated with blocking peptide #51246 (right).

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Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using p44/42 MAP Kinase (Phospho-Tyr204) Antibody #11246.

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Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody #11246.

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Western blot analysis of various lysates using p44/42 MAP Kinase(Phospho-Tyr204) Antibody at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (L3012) at 1:10000 dilution.Lysates/proteins: 25μg per lane.