- 询价
- Signalway Antibody
- 33191
- 2025年09月03日
- WB IHC IF
- Rabbit
- Human;Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthesized non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 312 (A-T-S(p)-P-A).
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB IHC IF
- 靶点：
Uniprot：P35568
- 抗体英文名：
IRS-1 Antibody
- 规格：
50ul/100ul
配方:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
图片:
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IRS-1 (Ab-312) antibody #33191.
,
Immunofluorescence analysis of HeLa cells, using IRS-1 (Ab-312) antibody #33191.
,
Western blot analysis of extracts from NIH-3T3 cells, using IRS-1 (Ab-312) antibody #33191.
,
Western blot analysis of extracts from JK cells (Lane 2), using IRS-1 (Ab-312) antiobdy #33191. The lane on the left is treated with synthesized peptide.
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文献和实验Bacterial Expression of IRS-1 containing GST-fusion Proteins
1. Grow cells in 5ml (or more) of LB-Amp overnight for a starter culture. 2. Grow larger culture using the overnight culture as a seeding culture. The culture is grown in LB-Amp medium. Aerate well with shaking at 37 C until OD at 600nm
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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