- 询价
- Signalway Antibody
- 11305
- 2025年09月03日
- WB，IHC，IF
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of tyrosine 199(Q-K-Y(p)-T-V)derived from Human IKK-β.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB，IHC，IF
- 靶点：
Uniprot：O14920
- 抗体英文名：
IKK-β(Phospho-Tyr199) Antibody
- 规格：
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
图片:
Immunofluorescence staining of methanol-fixed HeLa cells using IKK-β (Phospho-Tyr199) Antibody (#11305, Red).
,
Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml+Calyculin A 50nM 5', using IKK-beta (Phospho-Tyr199) Antibody. The lane on the right is blocked with the phospho peptide.
,
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IKK-beta (Phospho-Tyr199) Antibody. The picture on the right is blocked with the phospho peptide.
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity
Staining Whole Mouse Embryos for β-Galactosidase (lacZ ) Activity Andras Nagy , Marina Gertsenstein , Kristina Vintersten , and Richard Behringer This protocol was adapted from "Techniques for Visualizing Gene Products, Cells, Tissues
Imaging Gene Expression Using Antibody Probes
either β-galactosidase or green fluorescent protein (GFP), are physically labeled with dextran coupled to a fluorophore (8 ) or are labeled with fluorescent lipophilic dyes, such as diI (9 ). Multiple labeling experiments are then performed comparing
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