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- 文献和实验
- 技术资料
- 供应商:
BD Pharmingen
- 靶点:
CD5
- 级别:
RUO
- 克隆性:
单克隆
- 抗原来源:
Human
- 抗体英文名:
CD5 Antibody
- 抗体名:
CD5
- 标记物:
APC
- 宿主:
Mouse
- 适应物种:
Human
- 免疫原:
Human T cells
- 亚型:
Mouse BALB/c IgG1, κ
- 形态:
液体
- 应用范围:
Flow Cytometry (FCM)
- 保存条件:
2-8℃避光保存
- 规格:
25 Tests
产品概述
Hu CD5 APC UCHT2 25Tst 为 BD Pharmingen 品牌流式抗体,货号 561003,靶向 CD5,荧光标记 APC,克隆 UCHT2 (RUO),规格 25 Tests。适用于 Flow cytometry (Routinely Tested) 检测,仅供科研使用(RUO)。
靶点与抗体说明
The UCHT2 monoclonal antibody specifically binds to CD5. CD5 is a 67 kDa single-chain, type 1 transmembrane glycoprotein expressed on most thymocytes, the majority of peripheral T lymphocytes and a subpopulation of B cells. CD72 has been shown to be the natural ligand for CD5. CD5+ B cells produce polyreactive antibodies (mostly IgM).
核心参数
| 货号 | 561003 |
| 品牌 | BD Pharmingen |
| 克隆号 | 克隆 UCHT2 (RUO) |
| 宿主 | Mouse |
| 同种型 | Mouse BALB/c IgG1, κ |
| 荧光标记 | APC |
| 抗原 / 靶点 | CD5 |
| 反应种属 | Human |
| 验证应用 | Flow cytometry (Routinely Tested) |
| 推荐用量 | 20 µl |
| 免疫原 | Human T cells |
| 规格 | 25 Tests |
| 存储缓冲液 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
荧光染料信息
APC Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye. 格式:APC最多激发源:651 nm最多发射源:660 nm
保存与运输
建议 2-8℃ 避光保存,请勿冷冻,避免反复冻融。 存储缓冲液:Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
参考文献
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182. Lankester AC, van Schijndel GM, Cordell JL, van Noesel CJ, van Lier RA. CD5 is associated with the human B cell antigen receptor complex. Eur J Immunol. 1994; 24(4):812-816. (Biology). Lydyard PM, Lamour A, MacKenzie LE, Jamin C, Mageed RA, Youinou P. CD5+ B cells and the immune system. Immunol Lett. 1993; 38(2):159-166. (Biology). McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:1-1050. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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文献和实验Isolation Kit CD5(Ly-1) MicroBeadsCD8a(Ly-2) MicroBeadsCD8a+ T Cell Isolation Kit CD25 MicroBead KitCD62L(L-selectin) MicroBeadsAnti-MHC class II MicroBeadsCD4-FITC/PE/APCCD8a-FITC/PE/APCCD25-PECD62L-FITC/PE/APCCD90-FITC/PE/APC细胞因子分泌细胞Mouse IFN-r Secretion
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
and one mAb directly conjugated to allophycocyanin (APC) for combining dual-color immunofluorescence with DNA-RNA staining. See Troubleshooting and Discussion for further information on strategies for fluorochrome selection and staining. Nucleic
Intracellular Cytokine Staining Protocol
, FITC, Cy5, PE, PE-Cy5, and APC conjugates. For more information about the properties of fluorescent dyes, please visit our Fluorescent Dyes page in our BestProtocols. The Functional Grade Purified format is recommended for neutralization studies
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