Hu PD-L2 (CD273) BV421 24F.10C

产品概述

Hu PD-L2 (CD273) BV421 24F.10C12 25Tst 为 BD Pharmingen 品牌流式抗体，货号 568312，靶向 CD273 (PD-L2)，荧光标记 BV421，克隆 24F.10C12 (RUO)，规格 25 Tests。适用于 Flow cytometry (Routinely Tested) 检测，仅供科研使用（RUO）。

靶点与抗体说明

The 24F.10C12 monoclonal antibody specifically recognizes Programmed Death Ligand 2 (PD-L2, PDL2) which is also known as CD273, and B7-DC (B7DC). PD-L2 (CD273) is a type 1 transmembrane glycoprotein that is encoded by PDCD1LG2 (programmed cell death 1 ligand 2) which belongs to the B7 family within the  Ig superfamily. PD-L2 (CD273) serves as a ligand for CD279, the Programmed Death 1 (PD-1) receptor. CD273 is expressed on dendritic cells, activated monocytes, medullary thymic epithelial cells, placental trophoblasts, and myocardial endothelium. PD-L2 (CD273) can function as a coinhibitor of T cell functions by binding to and signaling through CD279 (PD-1). PD-L2 (CD273) can also reportedly act through another receptor to costimulate T cell activation and cytokine production. The 24F.10C12 antibody can reportedly block the binding of PD-L2 (CD273) to CD279 (PD-1).

核心参数

荧光染料信息

BV421 The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

保存与运输

建议 2-8℃ 避光保存，请勿冷冻，避免反复冻融。 存储缓冲液：Aqueous buffered solution containing ≤0.09% sodium azide.

参考文献

Brown JA, Dorfman DM, Ma FR, et al. Blockade of programmed death-1 ligand on dendritic cells enhances T cell activation and cytokine production. J Immunol. 2003; 170:1257-1266. (Immunogen: Blocking, Flow cytometry, Functional assay, Immunohistochemistry). Messal N, Serriari NE, Pastor S, Nunès JA, Olive D. PD-L2 is expressed on activated human T cells and regulates their function.. Mol Immunol. 2011; 48(15-16):2214-9. (Clone-specific: Flow cytometry, Stimulation). Rodig N, Ryan T, Allen JA, et al. Endothelial expression of PD-L1 and PD-L2 down-regulates CD8+ T cell activation and cytolysis.. Eur J Immunol. 2003; 33(11):3117-26. (Clone-specific: Blocking, Flow cytometry, Functional assay). Tavukcuoglu E, Horzum U, Yilmaz KB, Esendagli G. PD-L2+ wound zone macrophage-like cells display M1/M2-mixed activation and restrain the effector Th1 responses.. Immunol Cell Biol. 2020; 98(2):152-164. (Clone-specific: Flow cytometry).