Ms CD226 PE-Cy7 10E5 50ug

产品概述

Ms CD226 PE-Cy7 10E5 50ug 为 BD Pharmingen 品牌流式抗体，货号 567651，靶向 CD226，荧光标记 PE-Cy7，克隆 10E5 (RUO)，规格 50 ug。适用于 Flow cytometry (Routinely Tested) 检测，仅供科研使用（RUO）。

靶点与抗体说明

The 10E5 monoclonal antibody specifically binds to CD226 which is also known as, Platelet and T-cell activation antigen 1 (Pta1), T lineage-specific activation antigen 1 (TLiSA1), or DNAX accessory molecule-1 (DNAM-1). CD226 is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. CD226 serves as an adhesion molecule that is primarily expressed on T lymphocytes. Naïve CD8+ T cells express high levels of CD226 whereas it is differentially expressed on CD4+ T cells. In certain model systems, long term-cloned Th1 cells can express high CD226 levels when compared with the downregulated CD226 expression exhibited on cloned Th2 and Th0 cells. CD226 expression is also detectable on subsets of CD11b+ macrophages and NK cells. Adhesive interactions between CD226 and its ligands, CD112 and CD155, can result in cell signaling events that promote innate and adaptive immune responses, including the differentiation and survival of cytotoxic cells.

核心参数

荧光染料信息

PE-Cy7 PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye. 格式:PE-Cy7最多激发源:496 nm, 566 nm最多发射源:781 nm

保存与运输

建议 2-8℃ 避光保存，请勿冷冻，避免反复冻融。 存储缓冲液：Aqueous buffered solution containing ≤0.09% sodium azide.

参考文献

Dardalhon V, Schubart AS, Reddy J, et al. CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. Immunology. 2005; 175(3):1558-1565. (Immunogen: Flow cytometry, Inhibition, In vivo exacerbation). Seth S, Georgoudaki AM, Chambers BJ et al. Heterogeneous expression of the adhesion receptor CD226 on murine NK and T cells and its function in NK-mediated killing of immature dendritic cells. J Leukoc Biol. 2009; 86(1):91-101. (Biology). Tahara-Hanaoka S, Shibuya K, Kai H, et al. Blood. 2006; 107(4):491-496. (Biology). Tahara-Hanaoka S, Shibuya K, Onoda Y et al. Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112). Int Immunol. 2006; 16(4):533-538. (Biology).