Hu/NHP TNF PE-Cy7 MAb11 50Tst

产品概述

Hu/NHP TNF PE-Cy7 MAb11 50Tst 为 BD Pharmingen 品牌流式抗体，货号 560678，靶向 TNF，荧光标记 PE-Cy7，克隆 MAb11 (RUO)，规格 50 Tests。适用于 Intracellular staining (flow cytometry) (Routinely Tested) 检测，仅供科研使用（RUO）。

靶点与抗体说明

The MAb11 monoclonal antibody specifically binds to human tumor necrosis factor (TNF, also known as TNF-α) protein. TNF is an efficient juxtacrine, paracrine and endocrine mediator of inflammatory and immune functions. It regulates the growth and differentiation of a variety of cell types. TNF is cytotoxic for transformed cells when in conjunction with IFN-γ. It is secreted by activated monocytes/macrophages and other cells such as B cells, T cells and fibroblasts. The immunogen used to generate the MAb11 hybridoma was recombinant human TNF. The MAb11 antibody has been reported to crossreact with Rhesus Macaque TNF. MAb11 has been predicted to be reactive on other non-human primate samples (e.g Baboon, Cynomolgus). Investigators are advised that the PE-Cy™7 Mouse Anti-Human TNF (clone MAb11) antibody is not routinely tested on these other non-human primate samples.

核心参数

荧光染料信息

PE-Cy7 PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye. 格式:PE-Cy7最多激发源:496 nm, 566 nm最多发射源:781 nm

保存与运输

建议 2-8℃ 避光保存，请勿冷冻，避免反复冻融。 存储缓冲液：Aqueous buffered solution containing BSA and ≤0.09% sodium azide.