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产品概述 APC 标记大鼠抗小鼠 MHC Class II 单克隆抗体,克隆号 NIMR-4,特异性识别小鼠 MHC II 类分子,适配流式细胞检测。
应用方向 MHC II 分子主要表达于抗原提呈细胞表面,参与抗原提呈过程,常用于小鼠树突状细胞、巨噬细胞、B 细胞等免疫细胞亚群鉴定与活化状态分析,APC 荧光信号稳定,适配多色流式组合方案。
试剂特性 缓冲体系:pH7.2–7.4 磷酸盐溶液,含 0.1% ProClin 300、0.2% BSA,无额外修饰。 染色用量:5 μl / 测试。
储存提示 4℃避光保存,禁止冻存,有效期 12 个月。
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文献和实验Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules
binding peptides. We present here a high content screening for MHC class II (HLA-DR) binding to a peptide library in a chip-format. Binding of soluble MHC class II molecules to individual peptides can be visualized using an anti-DR directed monoclonal
), and then to deliver the peptide-MHC complexes to the plasma membrane in order to stimulate T cells. It is becoming increasingly clear that most of the intracellular compartments that contain MHC class II products in APC simply represent the conventional endosomes
Studying MHC class II Presentation of Immobilized Antigen by B Lymphocytes
. This allows T-B cooperation, a cellular communication that is required for B cells to develop into germinal centers (GC) and form mature high-affinity antibody producing cells and to further develop B cell memory. MHC class II antigen presentation by B
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