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文献和实验In Vitro Selection of Peptides Acting on NMDA Receptors
phage particles with few or many copies of peptide-bearing pVIII relative to wild-type pVIII. Libraries were affinity-selected against a soluble form of the extracellular amino-terminal domain of NR1 produced by the method described in Chapter
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
the focus systematically up and down in 1 um steps and triggering the ablation laser. The expanded, aligned, and convergence adjusted beam should ablate maximally at the image focus and weakly or not at all 1 um above or below focus (fig 5). 13)
Construction and Characterization of Adenovirus Vectors
. First-generation Ads have a large cloning capacity (5-14 kbp) and efficiently transduce a wide range of both quiescent and proliferating cell types. They are readily propagated to produce high-titer stocks (1011 -1013 vector particles from a 3-L culture
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