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- 文献和实验
- 技术资料
- 抗体名:
磷酸化丝裂原活化蛋白激酶p38α抗体
- 抗体英文名:
Anti-Phospho-p38 MAPK-T180/Y182 Monoclonal Antibody
- 靶点:
Phospho-p38 MAPK-T180/Y182
- 浓度:
WB 1:1000-2000. IHC-P 1:500-1000. IF-P 1:500-1000.
- 应用范围:
WB ; IHC-P ; IF-P
- 宿主:
Rabbit
- 适应物种:
Rat;Mouse;Human
- 保质期:
Store at -20℃. Avoid freeze / thaw cycles.
- 抗原来源:
A synthetic peptide of human Phospho-p38(Thr180/Tyr182)
- 目录编号:
1432
- 级别:
实验级
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- 标记物:
Biotin,Cy3,Cy3.5,Cy5,Cy5.5,Cy7,FITC,HRP,RBITC,SAlexa Fluor 350,SAlexa Fluor 405,SAlexa Fluor 488,SAlexa Fluor 532,SAlexa Fluor 555,SAlexa Fluor 568,SAlexa Fluor 594,SAlexa Fluor 633,SAlexa Fluor 640,SAlexa Fluor 647,SAlexa Fluor 660,SAlexa Fluor 680,SAlexa Fluor 750
- 克隆性:
Monoclonal Antibody
- 保存条件:
Store at -20℃. Avoid freeze / thaw cycles.
- 形态:
干粉/液体
- 亚型:
IgG, κ
- 免疫原:
A synthetic peptide of human Phospho-p38(Thr180/Tyr182)
- 规格:
100ul
【抗体靶点简介】
P38 MAP kinase is the mammalian homologue of the yeast HOG kinase and participates in a cascade controlling cellular responses to cytokines and stress. Like the SAPK/JNK pathway, P38 MAP kinase is activated by a variety of cellular stresses including inflammatory cytokines, UV light and growth factors, etc. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase-2 and to phosphorylate the transcription factors ATF-2 and Max.【实验操作视频参考】
![]() Western blot实验参考操作流程 | ![]() Immunohistochemistry实验参考操作流程 |
抗体在科研领域应用十分广泛,对解析生物学过程、探究疾病发病机制、创新药物研发与临床治疗研究均起到关键支撑作用。
索莱宝现有30000余种经过严格实验验证的抗体产品,全面覆盖神经科学、细胞凋亡、表观遗传学等热门研究方向,适配 WB、IHC、ELISA、IP、co-IP 等主流实验技术场景。包括内参标签抗体,以及多种标记类型的一抗、二抗,充分满足客户多样化实验选型需求。索莱宝拥有成熟专业的抗体制备与质量检测平台,从免疫制备、纯化提纯到实验验证全流程严格筛选评估,全方位保障每一支抗体的性能与品质。
此外,索莱宝已完善一站式技术服务平台,可提供 WB、IHC、IF、IP、流式检测、细胞实验等常规检测,以及多克隆/单克隆抗体定制等个性化定制服务,为科研用户提供全方位解决方案。


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文献和实验Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
largely independent of the surrounding amino-acid sequence. The antibody is phospho-specific but does not cross-react with phospho-serine-containing sequences. The anti-phosphothreonine monoclonal antibody is labeled with Eu3+ Cryptate by Cisbio
4. Remove solution and rinse ovaries with PBS 3 times, then wash 3 times for 10 minutes each 5. It may help antibody penetration to dissect apart the egg chambers 6. Incubate ovaries in PBT for 10 minutes 7. Add monoclonal supernatant
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