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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Mouse anti-c-Jun Monoclonal mAb(10G2)
- 抗体英文名:
Mouse anti-c-Jun Monoclonal Antibody(10G2)
- 靶点:
见优宁维官网
- 浓度:
见优宁维官网
- 应用范围:
IHC: 1:50-1:100
- 宿主:
Mouse
- 适应物种:
见优宁维官网
- 保质期:
见优宁维官网
- 抗原来源:
见优宁维官网
- 级别:
优级
- 库存:
999
- 供应商:
Absin
- 标记物:
Unlabeled
- 克隆性:
10G2
- 保存条件:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 免疫原:
Synthetic peptide conjugated to KLH.
- 规格:
100uL
别名:鼠抗c-Jun单克隆抗体(10G2)
免疫原:Synthetic peptide conjugated to KLH.
应用:IHC: 1:50-1:100
保持条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
使用方法:IHC
标记:Unlabeled
宿主:Mouse
反应种属:Human,Rat,Mouse
克隆性:10G2
产品描述:见优宁维官网
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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