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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Rabbit anti-Histone H3 Polyclonal mAb(HRP)
- 抗体英文名:
Rabbit anti-Histone H3 Polyclonal Antibody(HRP)
- 靶点:
见优宁维官网
- 浓度:
见优宁维官网
- 应用范围:
WB: 1:5000-1:10000
- 宿主:
Rabbit
- 适应物种:
见优宁维官网
- 保质期:
见优宁维官网
- 抗原来源:
见优宁维官网
- 级别:
优级
- 库存:
999
- 供应商:
Absin
- 标记物:
HRP
- 克隆性:
见优宁维官网
- 保存条件:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 免疫原:
A synthetic peptide corresponding to the carboxy terminus of the human histone H3 protein.
- 规格:
100uL
别名:兔抗组蛋白H3多克隆抗体(HRP)
免疫原:A synthetic peptide corresponding to the carboxy terminus of the human histone H3 protein.
应用:WB: 1:5000-1:10000
保持条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
使用方法:WB
标记:HRP
宿主:Rabbit
反应种属:Human,Mouse,Rat
克隆性:见优宁维官网
产品描述:见优宁维官网
爱必信品牌介绍:

更多详情请了解优宁维网站》》
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文献和实验我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
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