- ￥2300
- Absin
- abs158623
- 上海
- 2025年08月21日
- WB: 1:500-1:1000, IP: 1:20, IHC: 1:50-1:100, IF: 1:50-1:200, FCM: 1:50-1:100
- Rabbit
- 见优宁维官网
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Rabbit anti-Alkaline Phosphatase Polyclonal mAb
- 抗体英文名：
Rabbit anti-Alkaline Phosphatase Polyclonal Antibody
- 靶点：
见优宁维官网
- 浓度：
见优宁维官网
- 应用范围：
WB: 1:500-1:1000, IP: 1:20, IHC: 1:50-1:100, IF: 1:50-1:200, FCM: 1:50-1:100
- 宿主：
Rabbit
- 适应物种：
见优宁维官网
- 保质期：
见优宁维官网
- 抗原来源：
见优宁维官网
- 级别：
优级
- 库存：
999
- 供应商：
Absin
- 标记物：
Unlabeled
- 克隆性：
见优宁维官网
- 保存条件：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 免疫原：
A synthesized peptide derived from human Alkaline Phosphatase
- 规格：
100uL
别名：兔抗碱性磷酸酶多克隆抗体
免疫原：A synthesized peptide derived from human Alkaline Phosphatase
应用：WB: 1:500-1:1000, IP: 1:20, IHC: 1:50-1:100, IF: 1:50-1:200, FCM: 1:50-1:100
保持条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
使用方法：WB,IP,IHC,IF,FlowCytometry
标记：Unlabeled
宿主：Rabbit
反应种属：Human,Mouse,Rat
克隆性：见优宁维官网
产品描述：见优宁维官网
爱必信品牌介绍：
更多详情请了解优宁维网站》》
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文献和实验ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
antibody. 30 minutes. 5. Jet wash in tris/HC1 saline buffer pH 7.6. 6. Wash in buffer. 3 minutes. Steps 7-9 for POLYCLONAL Primary Antisera Only 7. Incubate in mouse anti-rabbit immunoglobulins. 30 minutes. 8. Jet wash in buffer.
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
5 above. Fixation for > 5 minutes can lead to decreased enzyme activity. STAINING FOR ALKALINE PHOSPHATASE ACTIVITY Phosphatase genes are useful reporter genes as several histochemical methods that yield precipitated, highly colored and/or electron
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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