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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S9 of human GSK3 beta protein. The exact sequence is proprietary.
- 保存条件:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
- 克隆性:
见优宁维官网
- 标记物:
Unlabeled
- 适应物种:
见优宁维官网
- 保质期:
见优宁维官网
- 抗原来源:
见优宁维官网
- 级别:
优级
- 库存:
999
- 供应商:
Absin
- 宿主:
Rabbit
- 应用范围:
WB: 1:500-1:1000, IP: 1:10-1:100, IHC: 1:50-1:100, IF: 1:50-1:200, ICC: 1:50-1:200
- 浓度:
见优宁维官网
- 靶点:
见优宁维官网
- 抗体英文名:
Rabbit anti-Phospho-GSK3 β(Ser9) Polyclonal Antibody
- 抗体名:
Rabbit anti-Phospho-GSK3 β(Ser9) Polyclonal mAb
- 规格:
100uL/50uL
| 规格: | 100uL | 产品价格: | ¥2300.0 |
|---|---|---|---|
| 规格: | 50uL | 产品价格: | ¥1500.0 |
别名:兔抗磷酸化GSK3β(Ser9)多克隆抗体KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S9 of human GSK3 beta protein. The exact sequence is proprietary.WB: 1:500-1:1000, IP: 1:10-1:100, IHC: 1:50-1:100, IF: 1:50-1:200, ICC: 1:50-1:200Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.WB,IP,IHC,IF,ICCUnlabeledRabbitHuman, Mouse, Rat, Bovine, Pig, Zebrafish
克隆性:见优宁维官网
产品描述:
Rabbit polyclonal antibody to GSK3 beta (Phospho-S9).
爱必信品牌介绍:

更多详情请了解优宁维网站》》
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文献和实验at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
Assays (See Note 9) 1. Pre-incubate anti-GSK3β (5 μg) monoclonal antibody withprotein G-Sepharose (400 μL of a 50% slurry) in kinasebuffer at 4°C for 1 h to conjugate the antibody to the proteinG-Sepharose beads. 2. Centrifuge the beads at 6,000× g
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