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- Absin
- 上海
- abs113796
- 2025年08月21日
- WB: 1:500-1:2000
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
见优宁维官网
- 保存条件：
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
- 克隆性：
见优宁维官网
- 标记物：
Unlabeled
- 适应物种：
见优宁维官网
- 保质期：
见优宁维官网
- 抗原来源：
见优宁维官网
- 级别：
优级
- 库存：
999
- 供应商：
Absin
- 宿主：
Mouse
- 应用范围：
WB: 1:500-1:2000
- 浓度：
见优宁维官网
- 靶点：
见优宁维官网
- 抗体英文名：
Mouse anti-PTEN Monoclonal Antibody(N-term)
- 抗体名：
Mouse anti-PTEN Monoclonal mAb(N-term)
- 规格：
50uL
别名：鼠抗PTEN单克隆抗体（N端）见优宁维官网WB: 1:500-1:2000Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.WBUnlabeledMouseHuman, Mouse, Rat
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
, 50 ml + NaOH, 1N, 100 µl-> heat to 37ѓC, add ddH2 O up to 250 ml 上一篇：Antibody Conjugation Protocol 下一篇：Expanding Hybridoma Clones
Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
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