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- 文献和实验
- 技术资料
- 抗体名:
Mouse anti-EGFR Monoclonal mAb(C-term) (688CT33.1.3)
- 抗体英文名:
Mouse anti-EGFR Monoclonal Antibody(C-term) (688CT33.1.3)
- 靶点:
见优宁维官网
- 浓度:
见优宁维官网
- 宿主:
Mouse
- 适应物种:
见优宁维官网
- 保质期:
见优宁维官网
- 抗原来源:
见优宁维官网
- 级别:
优级
- 库存:
999
- 供应商:
Absin
- 标记物:
Unlabeled
- 克隆性:
688CT33.1.3
- 保存条件:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
- 免疫原:
见优宁维官网
- 规格:
50uL
别名:小鼠抗EGFR单克隆抗体(C端) (688CT33.1.3)见优宁维官网WB: 1:8000Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.WBUnlabeledMouseHuman
克隆性:688CT33.1.3
产品描述:
Mouse Monoclonal Antibody (Mab)
爱必信品牌介绍:

更多详情请了解优宁维网站》》
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文献和实验Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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