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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Rabbit anti-UHRF1 Polyclonal mAb
- 抗体英文名:
Rabbit anti-UHRF1 Polyclonal Antibody
- 靶点:
见优宁维官网
- 浓度:
1mg/ml
- 宿主:
Rabbit
- 适应物种:
见优宁维官网
- 保质期:
见优宁维官网
- 抗原来源:
见优宁维官网
- 级别:
优级
- 库存:
999
- 供应商:
Absin
- 标记物:
见优宁维官网
- 克隆性:
见优宁维官网
- 保存条件:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
- 免疫原:
A synthesized peptide derived from Human UHRF1(Accession Q96T88), corresponding to amino acid residues I32-D60.
- 规格:
50ug///100ug
别名:兔抗UHRF1多克隆抗体A synthesized peptide derived from Human UHRF1(Accession Q96T88), corresponding to amino acid residues I32-D60.WB: 1:500-1:2000, IP: 1:20-1:50, IHC: 1:50-1:200, IF: 1:20-1:50, ELISA: 1:20000-1:40000, CHIP: 1:20-1:50Store at -20 °C for one year. Avoid repeated freeze/thaw cyclesChIP,Elisa,FlowCytometry,ICC,ICC-IF,IF,IHC,IP,WB,other见优宁维官网RabbitHuman;Mouse;Rat
克隆性:见优宁维官网
产品描述:
UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5‘-CCAAT-3‘ box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1‘s ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2.
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Polyclonal Antibody Production
Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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