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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Rabbit anti-Akt Polyclonal mAb
- 抗体英文名:
Rabbit anti-Akt Polyclonal Antibody
- 靶点:
见优宁维官网
- 浓度:
1mg/ml
- 宿主:
Rabbit
- 适应物种:
见优宁维官网
- 保质期:
见优宁维官网
- 抗原来源:
见优宁维官网
- 级别:
优级
- 库存:
999
- 供应商:
Absin
- 标记物:
Unlabeled
- 克隆性:
见优宁维官网
- 保存条件:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
- 免疫原:
A synthesized peptide derived from Human AKT1(Accession P31749), corresponding to amino acid residues T435-C460.
- 规格:
100ug///50ug
别名:兔抗Akt多克隆抗体A synthesized peptide derived from Human AKT1(Accession P31749), corresponding to amino acid residues T435-C460.WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:100-1:500, ICC: 1:100-1:500, ELISA(peptide): 1:20000-1:40000Store at -20 °C for one year. Avoid repeated freeze/thaw cyclesWB,IHC,IF,ICC,ELISAUnlabeledRabbitHuman;Mouse;Rat;Monkey
克隆性:见优宁维官网
产品描述:
an AGC kinase that plays a critical role in controlling the balance between survival and AP0ptosis. Phosphorylated and activated by PDK1 in the PI3 kinase pathway.
爱必信品牌介绍:

更多详情请了解优宁维网站》》
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
米宝 这俩天做实验,极其郁闷。我做的总AKt和pAkt,大鼠脑组织,第一次跑总AKT的时候,我觉得可能是因为蛋白是提取的浓度比较好吧,出来了一条浓浓的条带,三组总AKt都出了,接下来做pAkt,就出了一个带,效果挺明显的。后来再做pAkt的时候,跑了4张膜,显影的时候,太干净了,上面什么都没有,甚至连个杂带,斑点 都没!实验是在实验员的领导下完成的,技术上基本没什么纰漏,查阅本版面有关这问题方面的介绍,有的解释说pakt降解了!一抗是用的Phospho-Akt
Polyclonal Antibody Production
Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed
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