【干冰】Mycoplasma TaqMan PCR Lyop

TaqMan Primer and Probe Design

Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs

Protocol for the Detection of Mycoplasma genitalium by PCR from Clinical Specimens and Subsequent Detection of Macrolide Resistance-Mediating Mutation

evidence that it is causing pelvic inflammatory disease (PID). Nucleic acid amplification tests are currently the only available methods for detection, but no commercially available tests have been thoroughly evaluated. Here we describe a TaqMan-based PCR

Mapping, PCR DNA Sequencing

-Multiplex PCR -GeneScan (ABI) -PCR related technologies -Primer extension -Taqman (ABI) -Orchid -Pyrosequencing -Ligase chain reaction -Oligos for microarrays GCG PRIME The GCG program PRIME is a good tool for the design of primers for PCR