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- Norgen
- NGB-TM31510L
- 2025年08月21日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
【干冰】HPV High and Low Risk TaqMan Lyophilized Probe/Primer and Control Set
- 供应商：
广州市左克
- 规格：
100Reactions
产品简介:
品牌：Norgen
货号：NGB-TM31510L
产品名称：【干冰】HPV High and Low Risk TaqMan Lyophilized Probe/Primer and Control Set
产品规格：100Reactions
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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
sample DNA templates were amplified at different annealing temperatures as shown: note that while plasmid is amplified from 37 to 55℃, HPV DNA is only specifically amplified at 50℃.Primer Length The optimum length of a primer depends upon its （A+T
that have abolished RNase H activity in real-time RT-PCR experiments. Unlike many other qRT-PCR kits, MessageSensor includes a total RNA control, a control human GAPDH primer set, RNase inhibitor, and nucleotides, as well as a buffer additive that enables detection
: The most critical temperatures are those for annealing and denaturation. An annealing temperature that is too low results in non-specific amplifications. One too high results in no products or a low yield of product. Denaturation temperatures that are too low
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