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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
【干冰】16S V7-V9 Library Preparation Kit for Illumina (Set C)
- 供应商:
广州市左克
- 规格:
96Preps
产品简介:
品牌:Norgen
货号:NGB-70930
产品名称:【干冰】16S V7-V9 Library Preparation Kit for Illumina (Set C)
产品规格:96Preps
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
, including the Genome Analyzer (Solexa/Illumina), 454 (Roche), and ABI-SOLiD (Applied Biosystems). This protocol describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using
). 2. Stacking buffer (4×): 0.5 M Tris-HCl, pH 6.8, and 0.4%(w/v) SDS. Store at RT. 3. 30% acrylamide/bis solution (37.5:1 with 2.6% C) andN , N , N , N ¢-tetramethyl-ethylenediamine (TEMED; Bio-Rad,Hercules, CA). 4. Ammonium persulfate: prepare 10% (w/v
v468 Chapter 9 自身启动子报告基因验证转录靶点
/bacmids used): Ampicillin at 100 μg/mL medium, kanamycin at 25 μg/mLmedium, and chloramphenicol at 12.5 μg/mL medium. 4. Buffer P1, P2, and P3 taken from an arbitrary Qiagen DNAIsolation Kit (Qiagen, Valencia, CA ). 5. Isopropanol. 6. 70% (v/v
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