EXTRAClean RNA Clean-Up and Co

Using Soil DNA Kit (D5625) to Clean Up DNA containing inhibitors

with downstream applications. 13. Place column into a clean 1.5 mL microcentrifuge tube (not supplied). To elute DNA add 30 ìL-50 ìL of Elution Buffer (10 mM Tris buffer, pH 8.5) directly onto the center of HiBind matrix® . Incubate at 65EC

PureLink RNA Mini Kit

-stator homogenizer at maximum speed for ≥45 s. Centrifuge the homogenate at 26,000 × g for 5 minutes, then transfer the supernatant to a clean RNase-free tube.                       v.              Proceed to RNA Purification 2.        RNA

Isolation of micro-RNA (miRNA)

  Adapted from the Instruction manual of Stratagene''s Micro RNA Isolation Kit (Catalog #200344) Overview There are two main methods for isolating RNA: phenol-based extraction and silica matrix or glass fiber filter (GFF