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文献和实验Preparation of probes for in situ hybridization
in the sequence. These free amines are chemically coupled to fluorophores after synthesis. The modified Ts should be about 10 bases apart to prevent quenching and high background. The GC content of the oligo should be around 50%, its length can be variable, but 40
Preparation of probes for in situ hybridization
after synthesis. The modified Ts should be about 10 bases apart to prevent quenching and high background. The GC content of the oligo should be around 50%, its length can be variable, but 40-50 bases (5 fluorochromes per molecule of probe) works well in most cases
Pollen Development in Anthers of Arabidopsis
in Petri dishes on a layer of sterilized superfine grade Perlite pre-wetted with Gamborg's B5 Medium (see Growing Arabidopsis protocol). 2. Incubate in a growth chamber at 19-23°C under cool white fluorescent light (1500 to 2200 lux).
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