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文献和实验Pollen Development in Anthers of Arabidopsis
in Petri dishes on a layer of sterilized superfine grade Perlite pre-wetted with Gamborg's B5 Medium (see Growing Arabidopsis protocol). 2. Incubate in a growth chamber at 19-23°C under cool white fluorescent light (1500 to 2200 lux).
Preparation of probes for in situ hybridization
7 or Sp6 RNA polymerases. The fluorescent label can be incorporated during transcription (following manufacturer indications), by using nucleotides that are already coupled to fluorophores. Unfortunately, these nucleotides are incorporated
Preparation of probes for in situ hybridization
kit per reaction, Amersham), works very well for as a red fluorophore, and the succinimidyl ester of Oregon green 488 (1mg of compound per reaction, Molecular Probes) as a green dye. Labeling is then conducted for 24-48 h in the dark, at room
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